Selected Methods / Model Organisms
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Purification of Proteins, RNA, DNA | ||
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Primer extension, 5', 3' RACE |
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In vitro transcription | ||
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Southern, Northern, Western blotting | ||
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Promoter activity in vivo | ||
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Protein - GFP fusions | ||
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ATPase, GTPase Assays | ||
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Site directed mutagenesis | ||
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Nucleotide Binding Assay | ||
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Immunoprecipitation | ||
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RT-qPCR | ||
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Protein-protein interactions | ||
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RNA-protein interactions | ||
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Cross-linking studies | ||
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Creation of knock outs/mutationos of/in gene | ||
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RNAseq, ChIPseq | ||
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Bacillus subtilis, Escherichia coli, Mycobacterium smegmatis |